[Permanent link] [Permanent link] Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues

[Prev] [Prev]

Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues

[Next] [Next]
[Full screen] [Full screen]
« ‹ Prev
Next › »
 Index 
High throughput, large field of view structural imaging with SCAPE 2.0 Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues Shooting stars enlighten intrathalamic plasticity Quadruple whole-cell patch clamp in visual cortex Loss of Dickkopf-1 restores neurogenesis in old age and counteracts cognitive decline Expression of XFP in the Hippocampus and Cerebral Cortex CLARITY mouse hippocampus Neuronal culture Hippocampal neurons (green) and glial cells (red) Differentiation of cholinergic amacrine cells during chick embryonic development Horizontal cell in piriform cortex of mouse Neurogliaform cell in piriform cortex of mouse Mouse amygdala Simultaneous quadruple patch-clamp recording from layer 5 pyramidal neurons in a cortical brain slice Mouse olfactory bulb P2Y1 receptor localization in the neonatal rat cerebellum Ganglion in the myenteric plexus of a mouse Differentiation of cholinergic amacrine cells during chick embryonic development Cortical neuron culture transfected with EGFP Ex vivo cortical electroporation Mouse embryo axons Immunofluorescent colocalization of Oxytocin and Tyrosine Hydroxylase in the Parventricular Nucleus of a steroid treated hamster Mouse amygdala neurons activated by fear stimulus Brainbow Mouse Hippocampus Brainbow cerebellum Brainbow cortex Brainbow hippocampus (dentate gyrus) Brainbow Mouse Brain Stem
Animated BrainBow CUBIC-HV Fluorescent  × Myelin Populations  × Recording and Manipulation Single Cells Synapses

Zoom:

Remove cookie

Legend

CUBIC-HV allows multichannel whole-organ labeling. BOBO-1, PV (Cy3), Sst (A594), and Gad67 (A647) quadruple costaining images of the sole whole adult mousebrain reconstituted with Imaris software. Scale bar: 2 mm


Citation

Etsuo A. Susaki, Chika Shimizu, Akihiro Kuno, Kazuki Tainaka, Xiang Li, Kengo Nishi, Ken Morishima, Hiroaki Ono, Koji L. Ode, Yuki Saeki, Kazunari Miyamichi, Kaoru Isa, Chihiro Yokoyama, Hiroki Kitaura, Masako Ikemura, Tetsuo Ushiku, Yoshihiro Shimizu, Takashi Saito, Takaomi C. Saido, Masashi Fukayama, Hirotaka Onoe, Kazushige Touhara, Tadashi Isa, Akiyoshi Kakita, Mitsuhiro Shibayama and Hiroki R. Ueda (2020). Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues. Nature Communications 11:1982. DOI:10.1038/s41467-020-15906-5


Location

Japan


Technique

CUBIC-HV


Press release

Press release (RIKEN)