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Aplysia neuron

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[more] [more] Transistor Probes Local Potassium Conductances in the Adhesion Region of Cultured Rat Hippocampal Neurons Dentate gyrus granule neurons Adult mouse hippocampus Osmium tetroxide stained transmission electron micrograph of the many layers of membrane in myelin Hippocampal growth cones making contact Two hippocampal growth cones making a connection Hippocampal neuron growth cone pathfinding Axons forming a glomerulus in a mouse olfactory bulb Biocytin stained layer Va pyramidal neurons in rat barrel cortex Synaptic connection between an interneuron and a postsynaptic Purkinje cell in the cerebellar cortex Golgi Stained Pyramidal Cell Neuron from the abdominal ganglia of <i>Aplysia limacina</i> immunostained with the antibody anti-D-aspartic acid Green Fluorescent Protein expressing pyramidal cell in mouse cortex Sensory nerve ending of hair follicle Cerebral pyramidal neurons (Golgi stain) Astrocytes (Golgi stain) Pyramidal neurons from the central nervous system growing on a glial / fibroblast matrix Backlabelled layer 5 pyramidal neurons Micrograph of neurons in rat neocortex Aplysia neuron Purkinje neuron in mouse cerebellum Cultured motor neuron (red) on a monolayer of astrocytes Astrocyte Hippocampal neurons (green) and glial cells (red) Hippocampal neuron expressing monomeric Green Fluorescent Protein Neonatal rat pyramidal neuron Pyramidal neurons in mouse cerebral cortex (Golgi stain) Differentiation of cholinergic amacrine cells during chick embryonic development PHF-tau-ir neurons PHF-tau-ir neurons - PV-ir axon terminals PHF-tau-ir neurons - chandelier cell axon terminals Hippocampal neuron stained for spectrin (red) and tubulin (green) Neuron transfected with doublecortin (DCX) DsRed and mannose phosphate receptor (MPR) GFP-1 Laser confocal images of neurons in hippocampal slice gene gun transfected with DCX DsRed and μ1A GFP Horizontal cell in piriform cortex of mouse Neurogliaform cell in piriform cortex of mouse Mouse amygdala Simultaneous patch-clamp recording from a cortical pyramidal neuron Microelectrode Dye in Purkinje Cell [more] [more]
Animated BrainBow CUBIC-HV Fluorescent Myelin Populations Recording and Manipulation Single Cells Synapses

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Second-harmonic generation microscopy image of a primary cultured Aplysia neuron stained with the membrane dye DHPESBP. The signal is modulated by membrane potential and was found to be capable of recording action potentials with 0.6 μm and 0.833 msec spatiotemporal resolution. The high-resolution and deep tissue imaging capability of this nonlinear microscopy technique should prove valuable to future electrophysiology studies.


Technique

Second harmonic generation microscopy


Citation

Optical Recording of Action Potentials with Second-Harmonic Generation Microscopy, Daniel A. Dombeck et al., The Journal of Neuroscience, January 28, 2004, 24(4):999–1003; doi:10.1523/JNEUROSCI.4840-03.2004