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Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues

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High throughput, large field of view structural imaging with SCAPE 2.0 Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues Versatile whole-organ-body staining and imaging based on electrolyte-gel properties of biological tissues Shooting stars enlighten intrathalamic plasticity Brainbow mouse hippocampus Quadruple whole-cell patch clamp in visual cortex Loss of Dickkopf-1 restores neurogenesis in old age and counteracts cognitive decline Expression of XFP in the Hippocampus and Cerebral Cortex CLARITY mouse hippocampus Labeled ganglion cells in the mouse retina Neuronal culture Transistor Probes Local Potassium Conductances in the Adhesion Region of Cultured Rat Hippocampal Neurons Dentate gyrus granule neurons Adult mouse hippocampus Pyramidal neurons from the central nervous system growing on a glial / fibroblast matrix Backlabelled layer 5 pyramidal neurons Micrograph of neurons in rat neocortex Hippocampal neurons (green) and glial cells (red) Pyramidal neurons in mouse cerebral cortex (Golgi stain) Differentiation of cholinergic amacrine cells during chick embryonic development Horizontal cell in piriform cortex of mouse Neurogliaform cell in piriform cortex of mouse Mouse amygdala Simultaneous quadruple patch-clamp recording from layer 5 pyramidal neurons in a cortical brain slice Biocytin-labeled layer 5 pyramidal neurons from multiple recordings and Neurolucida reconstruction Biocytin-labeled interneurons and pyramidal neurons Golgi stained neurons in the dentate gyrus of an epilepsy patient Pyramidal neurons of mouse cortex SMI32-immunoreactive pyramidal neuron in medial prefrontal cortex of macaque Mouse olfactory bulb P2Y1 receptor localization in the neonatal rat cerebellum Ganglion in the myenteric plexus of a mouse Dissociated culture of hippocampal neurons Differentiation of cholinergic amacrine cells during chick embryonic development Cortical neuron culture transfected with EGFP Ex vivo cortical electroporation [more] [more]
Animated BrainBow CUBIC-HV Fluorescent Myelin Populations  × Recording and Manipulation Single Cells Synapses

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CUBIC-HV allows whole-organ cellular function analysis. The NMDA receptor antagonist MK-801 (or saline) was administered to wild-type mice. Whole-brain c-Fos antibody staining was then carried out using the CUBIC-HV method. The first and third panel show the saline-treated group (the right two panels have a filter applied to enhance visualisation). The second and fourth panel show the MK-801 treated group.


Citation

Etsuo A. Susaki, Chika Shimizu, Akihiro Kuno, Kazuki Tainaka, Xiang Li, Kengo Nishi, Ken Morishima, Hiroaki Ono, Koji L. Ode, Yuki Saeki, Kazunari Miyamichi, Kaoru Isa, Chihiro Yokoyama, Hiroki Kitaura, Masako Ikemura, Tetsuo Ushiku, Yoshihiro Shimizu, Takashi Saito, Takaomi C. Saido, Masashi Fukayama, Hirotaka Onoe, Kazushige Touhara, Tadashi Isa, Akiyoshi Kakita, Mitsuhiro Shibayama and Hiroki R. Ueda (2020). Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues. Nature Communications 11:1982. DOI:10.1038/s41467-020-15906-5


Location

Japan


Technique

CUBIC-HV


Press release

Press release (RIKEN)